Neurocognitive impairment in obstructive sleep apnea.

Obstructive sleep apnea syndrome (OSAS) is a typical dysfunction with far-reaching well being implications. One of many main penalties of OSAS is an impression on neurocognitive functioning. A number of research have proven that OSAS has an adversarial impact on inductive and deductive reasoning, consideration, vigilance, studying, and reminiscence.
Neurocognitive impairment might be measured objectively with exams such because the Wechsler Grownup Intelligence Scale-Revised, the Psychomotor Vigilance Job, the Steer Clear Efficiency Take a look at, and exams of repetitive finger tapping. In youngsters, OSAS could trigger attention-deficit hyperactivity dysfunction along with behavioral issues and studying disabilities.
Danger elements for cognitive impairment embody rising age, male intercourse, apolipoprotein E ε4 allele positivity, present cigarette smoking, weight problems, hypertension, diabetes mellitus, metabolic syndrome, Down syndrome, hypothyroidism, important alcohol consumption, stroke, and using psychoactive medicines. At a mobile stage, OSAS possible causes cognitive impairment by means of intermittent hypoxia, hormonal imbalance, and/or systemic irritation, both independently or through the resultant endothelial dysfunction that happens.
Extreme daytime sleepiness ought to be measured and minimized in all research of neurocognitive impairment. Current research have used useful and structural neuroimaging to delineate the mind areas affected in sufferers with OSAS with neurocognitive dysfunction.
A typical discovering in a number of of those research is decreased hippocampal quantity. Different affected mind areas embody the frontal and parietal lobes of the mind, which present focal reductions in grey matter. These adjustments might be reversed a minimum of partially with using CPAP, which highlights the significance of early recognition and remedy of OSAS. The at present out there information on this area are fairly restricted, and extra analysis is required.

Correlating chemical sensitivity and basal gene expression reveals mechanism of motion.

Adjustments in mobile gene expression in response to small-molecule or genetic perturbations have yielded signatures that may join unknown mechanisms of motion (MoA) to ones beforehand established. We hypothesized that differential basal gene expression might be correlated with patterns of small-molecule sensitivity throughout many cell traces to light up the actions of compounds whose MoA are unknown.
To check this concept, we correlated the sensitivity patterns of 481 compounds with ∼19,000 basal transcript ranges throughout 823 completely different human most cancers cell traces and recognized selective outlier transcripts. This course of yielded many novel mechanistic insights, together with the identification of activation mechanisms, mobile transporters and direct protein targets.
We discovered that ML239, initially recognized in a phenotypic display screen for selective cytotoxicity in breast most cancers stem-like cells, most probably acts by means of activation of fatty acid desaturase 2 (FADS2). These information and analytical instruments can be found to the analysis neighborhood by means of the Most cancers Therapeutics Response Portal.
Mathematical modeling of organic processes gives deep insights into complicated mobile programs. Whereas quantitative and steady fashions equivalent to differential equations have been broadly used, their use is obstructed in programs whereby the data of mechanistic particulars and kinetic parameters is scarce.
However, a wealth of molecular stage qualitative information on particular person elements and interactions might be obtained from the experimental literature and high-throughput applied sciences, making qualitative approaches equivalent to Boolean community modeling extraordinarily helpful.
On this paper, we construct on our analysis to supply a strategy overview of Boolean modeling in programs biology, together with Boolean dynamic modeling of mobile networks, attractor evaluation of Boolean dynamic fashions, in addition to inferring organic regulatory mechanisms from high-throughput information utilizing Boolean fashions.
We lastly show how Boolean fashions might be utilized to carry out the structural evaluation of mobile networks. This overview goals to acquaint life science analysisers with the fundamental steps of Boolean modeling and its purposes in a number of areas of programs biology.

Heme oxygenase-1 and the vascular mattress: from molecular mechanisms to therapeutic alternatives.

Heme oxygenase-1, an enzyme degrading heme to carbon monoxide, iron, and biliverdin, has been acknowledged as enjoying a vital function in mobile protection in opposition to worrying circumstances, not solely associated to heme launch. HO-1 protects endothelial cells from apoptosis, is concerned in blood-vessel rest regulating vascular tone, attenuates inflammatory response within the vessel wall, and participates in blood-vessel formation by the use of angiogenesis and vasculogenesis.
The latter features hyperlink HO-1 not solely to cardiovascular ischemia but additionally to many different circumstances that, like growth, wound therapeutic, or most cancers, are depending on neovascularization. The goal of this complete evaluate is to handle the mechanisms of HO-1 regulation and performance in cardiovascular physiology and pathology and to show some attainable purposes of the huge data generated to date.
Current information present highly effective proof for the involvement of HO-1 within the therapeutic impact of medication utilized in cardiovascular illnesses. Novel research open the probabilities of software of HO-1 for gene and cell remedy. Subsequently, analysis in forthcoming years ought to assist to elucidate each the true function of HO-1 within the impact of medication and the medical feasibility of HO-1-based cell and gene remedy, creating the efficient therapeutic avenues for this refined antioxidant system.

Meta-analysis of genome-wide affiliation research from the CHARGE consortium identifies widespread variants related to carotid intima media thickness and plaque.

Carotid intima media thickness (cIMT) and plaque decided by ultrasonography are established measures of subclinical atherosclerosis that every predicts future heart problems occasions. We performed a meta-analysis of genome-wide affiliation information in 31,211 members of European ancestry from 9 massive research within the setting of the Cohorts for Coronary heart and Growing older Analysis in Genomic Epidemiology (CHARGE) Consortium.
We then sought further proof to help our findings amongst 11,273 people utilizing information from seven further research. Within the mixed meta-analysis, we recognized three genomic areas related to widespread carotid intima media thickness and two completely different areas related to the presence of carotid plaque (P < 5 × 10(-8)).

T-Cell Receptor Delta Chain C Region (TRDC) Antibody

abx030770-400ul 400 ul
EUR 523

T-Cell Receptor Delta Chain C Region (TRDC) Antibody

abx030770-80l 80 µl
EUR 286

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 456
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 24-Well Cell Migration Assay (8 µm, Colorimetric Format), Trial Size

CBA-100-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (8 µm, Fluorometric Format), Trial Size

CBA-101-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-Well Cell Migration Assay (5 µm, Fluorometric Format), Trial Size

CBA-102-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 24-Well Cell Migration Assay (3 µm, Fluorometric Format), Trial Size

CBA-103-T 4 assays
EUR 299
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Colorimetric Format), Trial Size

CBA-110-T 4 assays
EUR 322
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 24-Well Cell Invasion Assay (Basement Membrane, Fluorometric Format), Trial Size

CBA-111-T 4 assays
EUR 322
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size

CBA-130-T 24 assays
EUR 386
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

Anti-Rat T-cell Receptor antibody

STJ16100718 1 mL
EUR 629

Human T- cell receptor delta chain C region, TRDC ELISA KIT

ELI-16511h 96 Tests
EUR 824

T-Cell Receptor Gamma-2 Chain C Region (TRGC2) Antibody

abx030771-400ul 400 ul
EUR 523

T-Cell Receptor Gamma-2 Chain C Region (TRGC2) Antibody

abx030771-80l 80 µl
EUR 286

CaMK2-beta / gamma / delta Cell ELISA Kit

abx595083-96tests 96 tests
EUR 637

Gamma-Aminobutyric Acid Receptor Subunit Delta (gABRD) Antibody

20-abx112666
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Gamma-Aminobutyric Acid Receptor Subunit Delta (gABRD) Antibody

abx034314-400ul 400 ul
EUR 523

Gamma-Aminobutyric Acid Receptor Subunit Delta (gABRD) Antibody

abx034314-80l 80 µl
EUR 286

Gamma-Aminobutyric Acid Receptor Subunit Delta (gABRD) Antibody

20-abx133715
  • EUR 314.00
  • EUR 154.00
  • EUR 439.00
  • EUR 258.00
  • 100 ul
  • 10 ul
  • 200 ul
  • 30 ul

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody

abx444998-100ug 100 ug
EUR 523

Gamma-Aminobutyric Acid Receptor Subunit Delta (gABRD) Antibody

abx233287-100ug 100 ug
EUR 509

Gamma-Aminobutyric Acid Receptor Subunit Delta (GABRD) Antibody

20-abx328382
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

TCR gamma / delta Antibody

abx139895-01mg 0.1 mg
EUR 314

TCR gamma / delta Antibody

abx140353-01mg 0.1 mg
EUR 356

TCR gamma / delta Antibody

abx140352-01mg 0.1 mg
EUR 356

TCR gamma / delta Antibody

20-abx200993
  • EUR 272.00
  • EUR 411.00
  • 100 ug
  • 500 ug

p38 gamma/delta Antibody

DF10341 200ul
EUR 304
Description: p38-delta/gamma Antibody detects endogenous levels of p38-delta/gamma.

gamma delta TCR antibody

10R-6534 100 ug
EUR 181
Description: Armenian Hamster monoclonal gamma delta TCR antibody

gamma delta TCR antibody

10R-6692 100 ug
EUR 208
Description: Mouse monoclonal gamma delta TCR antibody

TCR gamma delta antibody

10R-8120 100 ug
EUR 457
Description: Mouse monoclonal T Cell Receptor gamma delta antibody

T-Pro Stacking Buffer

JB03-C002 500ml/BT
EUR 152

T-Pro MycoClean spray

JT90-R002 500ml/BT
EUR 144

Anti-T-Cell receptor antibody

STJ16100480 100 µg
EUR 406

CaMK2-beta / gamma / delta (pT287) Cell ELISA Kit

abx596064-296tests 2 × 96 tests
EUR 707

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (ALP)

abx442395-100ug 100 ug
EUR 578

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (APC)

abx442676-100ug 100 ug
EUR 578

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (Biotin)

abx442956-100ug 100 ug
EUR 578

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (FITC)

abx443236-100ug 100 ug
EUR 565

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (HRP)

abx443517-100ug 100 ug
EUR 565

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (PerCP)

abx444079-100ug 100 ug
EUR 578

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (RPE)

abx444360-100ug 100 ug
EUR 578

Gamma-Aminobutyric Acid Receptor Subunit Delta (Gabrd) Antibody (Streptavidin)

abx444641-100ug 100 ug
EUR 578

Opioid Receptor-delta Cell ELISA Kit

abx595435-96tests 96 tests
EUR 637

CaMKIIBeta/Gamma/Delta Polyclonal Antibody

ABP56647-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of CaMKIIBeta/?/? from Human, Mouse, Rat. This CaMKIIBeta/?/? antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human CaMKII?/?/? around the non-phosphorylation site of T287

CaMKIIBeta/Gamma/Delta Polyclonal Antibody

ABP56647-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of CaMKIIBeta/?/? from Human, Mouse, Rat. This CaMKIIBeta/?/? antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human CaMKII?/?/? around the non-phosphorylation site of T287

CaMKIIBeta/Gamma/Delta Polyclonal Antibody

ABP56647-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of CaMKIIBeta/?/? from Human, Mouse, Rat. This CaMKIIBeta/?/? antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human CaMKII?/?/? around the non-phosphorylation site of T287

TCR gamma / delta Antibody (PE)

abx139901-01mg 0.1 mg
EUR 342

TCR gamma / delta Antibody (FITC)

abx140379-100tests 100 tests
EUR 425

TCR gamma / delta Antibody (PE)

abx140380-100tests 100 tests
EUR 481

CaMK2 beta / gamma / delta Antibody

abx011680-100ug 100 ug
EUR 439

TCR gamma / delta Antibody (APC)

20-abx200986
  • EUR 370.00
  • EUR 272.00
  • 100 ug
  • 25 ug

TCR gamma / delta Antibody (Biotin)

20-abx200987
  • EUR 314.00
  • EUR 258.00
  • 100 ug
  • 25 ug

TCR gamma / delta Antibody (FITC)

20-abx200989
  • EUR 286.00
  • EUR 244.00
  • EUR 398.00
  • 100 ug
  • 25 ug
  • 500 ug

TCR gamma / delta Antibody (PE)

20-abx200990
  • EUR 370.00
  • EUR 272.00
  • 100 ug
  • 25 ug

TCR gamma / delta Antibody (PerCP)

20-abx200991
  • EUR 481.00
  • EUR 286.00
  • 100 ug
  • 25 ug

CaMK2-beta/gamma/delta Antibody

AF6434 200ul
EUR 304
Description: CaMK2-beta/gamma/delta Antibody detects endogenous levels of total CaMK2-beta/gamma/delta.

p38 gamma/delta Blocking Peptide

DF10341-BP 1mg
EUR 195

gamma delta TCR antibody (FITC)

61R-1138 100 ug
EUR 316
Description: Armenian Hamster monoclonal gamma delta TCR antibody (FITC)

gamma delta TCR antibody (FITC)

61R-1144 50 ug
EUR 165
Description: Mouse monoclonal gamma delta TCR antibody (FITC)

gamma delta TCR antibody (PE)

61R-1342 100 ug
EUR 300
Description: Armenian Hamster monoclonal gamma delta TCR antibody (PE)

gamma delta TCR antibody (PE)

61R-1440 100 tests
EUR 403
Description: Mouse monoclonal gamma delta TCR antibody (PE)

gamma delta TCR antibody (biotin)

61R-1593 100 ug
EUR 316
Description: Armenian Hamster monoclonal gamma delta TCR antibody (biotin)

gamma delta TCR antibody (allophycocyanin)

61R-1772 100 ug
EUR 300
Description: Armenian Hamster monoclonal gamma delta TCR antibody (allophycocyanin)

CaMK2- beta/gamma/delta Antibody

ABF6434 100 ug
EUR 438

CaMK2 beta/gamma/delta antibody

70R-32640 100 ug
EUR 327
Description: Rabbit polyclonal CaMK2 beta/gamma/delta antibody

Human T- cell receptor gamma chain C region 1, TRGC1 ELISA KIT

ELI-51630h 96 Tests
EUR 824

Human T- cell receptor gamma- 2 chain C region, TRGC2 ELISA KIT

ELI-28913h 96 Tests
EUR 824

T-Cell Surface Glycoprotein CD3 Delta Chain (CD3D) Antibody

20-abx320335
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

T-Cell Surface Glycoprotein CD3 Delta Chain (CD3D) Antibody

20-abx133546
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

T-Cell Surface Glycoprotein CD3 Delta Chain (CD3D) Antibody

20-abx001150
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

T-Cell Surface Glycoprotein CD3 Delta Chain (CD3D) Antibody

20-abx009478
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

T-Complex Protein 1 Subunit Delta (TCP1 delta) Antibody

20-abx141878
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

Anti-Rat T-cell Receptor & Epithelial Tumours antibody

STJ16100721 1 mL
EUR 629

Recombinant T-Cell Surface Glycoprotein CD3 Gamma (CD3g)

4-RPD118Hu01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human T-Cell Surface Glycoprotein CD3 Gamma expressed in: E.coli

OxiSelect Nitrotyrosine ELISA Kit, Trial Size

STA-305-T 32 assays
EUR 421
Description: Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress. Our OxiSelect Nitrotyrosine ELISA Kit provides a sensitive method to measure the formation of 3-nitrotyrosine in proteins.

OxiSelect Protein Carbonyl Fluorometric Assay, Trial Size

STA-307-T 20 assays
EUR 351
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Fluorometric Assay Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The fluorescence plate-based format provides a convenient system for direct measurement of protein carbonyl content.

OxiSelect Protein Carbonyl Immunoblot Kit, Trial Size

STA-308-T 4 blots
EUR 264
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl Immunoblot Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The immunoblot format provides a convenient way to compare oxidized and non-oxidized protein fingerprints.

OxiSelect Protein Carbonyl ELISA Kit, Trial Size

STA-310-T 32 assays
EUR 421
Description: The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, lysine, arginine and threonine residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species. Our OxiSelect Protein Carbonyl ELISA Kit provides a rapid, efficient method for the detection of protein carbonyl residues. The ELISA format is perfect for higher throughput and high sensitivity; we have eliminated concentration and precipitation steps allowing greater sample retention.

OxiSelect Total Glutathione (GSSG/GSH) Assay Kit, Trial Size

STA-312-T 20 assays
EUR 282
Description: The OxiSelect Total Glutathione Assay Kit is a quantitative assay for measuring the total glutathione content within a sample (GSH/GSSG).  Glutathione Reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH) in the presence of NADPH.  Subsequently, the  chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm.  The total glutathione content in unknown samples is determined by comparison with the predetermined glutathione standard curve.  The rate of chromophore production is proportional to the concentration of glutathione within the sample.  The rate can be determined from the absorbance change over time.  Metaphosphoric acid is provided to remove interfering proteins or enzymes from samples.

OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size

STA-320-T 32 assays
EUR 508
Description: Among numerous types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the byproducts of oxidative DNA damage, is physiologically formed and enhanced by chemical carcinogens. Our OxiSelect Oxidative DNA Damage ELISA Kit (8-hydroxydeoxyguanosine assay) provides a powerful method for rapid, sensitive quantitation of 8-OHdG in DNA samples.

OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size

STA-321-T 20 assays
EUR 351
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation), Trial Size

STA-322-T 32 assays
EUR 351
Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures the formation of cyclobutane pyrimidine dimers (CPD) in DNA isolated from cells or tissues. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard are then probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation), Trial Size

STA-323-T 32 assays
EUR 351
Description: Our OxiSelect UV-Induced DNA Damage ELISA Kit measures 6-4PP formation in DNA isolated from cells or tissue. Standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA binding plate.  The sample or standard is then probed with an anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites), Trial Size

STA-324-T 10 assays
EUR 415
Description: DNA damage can manifest in the formation of apurinic or apyrimidinic (AP or abasic) sites. Such spontaneous base loss in mammalian cells has been estimated at between 50,000 and 200,000 sites per day. Unrepaired abasic sites can inhibit transcription and may be mutagenic. Our OxiSelect Oxidative DNA Damage Quantitation Kit provides a simple, user-friendly method for the quantitaiton DNA damage in the form of abasic sites. The assay uses an Aldehyde Reactive Probe (ARP) to specifically react with an aldehyde group on the open ring of the AP site. This allows the AP site to be labeled with Biotin, followed by detection with Streptavidin-Enzyme conjugate.

OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation), Trial Size

STA-325-T 32 assays
EUR 508
Description: Recently oxidative RNA damage has been described in conjunction with a wide variety of neurological diseases including Alzheimer?s disease, Parkinson?s disease, Dementia with Lewy Bodies, prion disease, and subacute sclerosing panencephalitis. We have developed an easy-to-use ELISA for the detection of the most common marker of RNA damage: 8-hydroxyguanosine (8-OHG).

OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD), Trial Size

STA-326-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit mesaures the formation of cyclobutane pyrimidine dimers (CPD) in intact cells. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by an HRP conjugated secondary antibody.

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD), Trial Size

STA-327-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (6-4PP), Trial Size

STA-328-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage ELISA Kit measures the formation of 6-4PP in intact cells in a 96-well ELISA plate-based format. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. 

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP), Trial Size

STA-329-T 32 assays
EUR 351
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect TBARS Assay Kit (MDA Quantitation), Trial Size

STA-330-T 20 assays
EUR 340
Description: The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid; the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect 8-iso-Prostaglandin F2a ELISA Kit, Trial Size

STA-337-T 32 assays
EUR 328
Description: 8-iso-Prostaglandin F2alpha (8-isoprostane) is a stable by-product of lipid peroxides generated during oxidative stress. Our OxiSelect 8-Isoprostane Assay Kit provides a convenient method for absolute quantitation in just a few hours. The 8-Isoprostane ELISA is suitable for a variety of sample types including urine, plasma, and cell lysates.

OxiSelect Catalase Activity Assay Kit, Fluorometric, Trial Size

STA-339-T 50 assays
EUR 398
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

OxiSelect Superoxide Dismutase Activity Assay, Trial Size

STA-340-T 20 assays
EUR 345
Description: Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidant enzymes. SOD enzymes are classified into three groups: cytosolic CuZn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. Our OxiSelect Superoxide Dismutase Activity Assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions and a chromagen to produce a water-soluble formazan dye upon reduction by superoxide anions. The superoxide dismutase activity is determined as the inhibition or reduction of chromagen.

OxiSelect Catalase Activity Assay Kit (Colorimetric), Trial Size

STA-341-T 20 assays
EUR 363
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kits measure catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 100 assays in a 96-well microtiter plate, or 50 assays in microcentrifuge tubes. This includes blanks, catalase standards and unknown samples. Direct spectrophotometric detection of catalase activity with ultraviolet light can cause interference from proteins and other biological components. The OxiSelect Catalase Activity Assay Kit (Colorimetric) utilizes visible light (520 nm), which reduces sample interference. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit (Fluorometric), Trial Size

STA-344-T 50 assays
EUR 287
Description: The OxiSelect Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase.   In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin.  The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm.  Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples.  The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.

OxiSelect Oxygen Radical Antioxidant Capacity (ORAC) Activity Assay, Trial Size

STA-345-T 48 assays
EUR 316
Description: The ORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect ORAC Activity Assay measures this capacity in less than 2 hours from a wide variety of sample types.

OxiSelect Hydroxyl Radical Antioxidant Capacity (HORAC) Activity Assay, Trial Size

STA-346-T 48 assays
EUR 316
Description: The HORAC assay is a powerful tool to measure the antioxidant capacity of biomolecules. Our OxiSelect HORAC Activity Assay measures the degradation of free hydroxyl radicals in less than 2 hours from a wide variety of sample types.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect Total Antioxidant Capacity (TAC) Assay Kit, Trial Size

STA-360-T 20 assays
EUR 282
Description: The OxiSelect Total Antioxidant Capacity (TAC) Assay measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. In the presence of antioxidants, copper(II) is reduced to copper(I). In turn, the copper(I) ions react with a chromogen to produce a color with maximum absorbance at 490nm.

Pan-Ras Activation Assay Kit, Trial Size

STA-400-T 5 assays
EUR 403
Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

Cdc42 Activation Assay Kit, Trial Size

STA-402-T 5 assays
EUR 403
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

Ral Activation Assay Kit, Trial Size

STA-408-T 5 assays
EUR 403
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay Kit, Trial Size

STA-409-T 5 assays
EUR 403
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Lipoprotein Lipase (LPL) Activity Assay Kit (Fluorometric), Trial Size

STA-610-T 20 assays
EUR 293
Description: The Lipoprotein Lipase (LPL) Activity Assay Kit is a simple, fluorometric assay that quantitatively measures LPL activity in plasma, serum, and lysates in a 96-well microtiter plate format.  Each kit provides sufficient reagents to perform up to 100 assays, including blanks, LPL standards, and unknown samples.  The kit contains a LPL Standard and has a detection sensitivity limit of ~1 mUnits/mL (see Kit Components for unit definition). Besides LPL, this assay can also be used to detect endothelial and hepatic lipase activity.

Lipid Extraction Kit (Chloroform-Free), Trial Size

STA-612-T 10 preps
EUR 270
Description: Cell Biolabs? Lipid Extraction Kit isolates total lipids by organic extraction, but circumvents disadvantages of traditional chloroform extraction methods by extracting lipids to an upper organic phase (making it amenable to high throughput extraction) that is chloroform free.

OxiSelect N?-(carboxymethyl) lysine (CML) Competitive ELISA Kit, Trial Size

STA-816-T 32 assays
EUR 421
Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.

OxiSelect Advanced Glycation End Product (AGE) Competitive ELISA Kit, Trial Size

STA-817-T 32 assays
EUR 398
Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. Our OxiSelect AGE Competitive ELISA kit is designed for rapid detection and quantitation of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.

OxiSelect MDA Adduct Competitive ELISA Kit, Trial Size

STA-832-T 32 assays
EUR 421
Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

OxiSelect HNE Adduct Competitive ELISA Kit, Trial Size

STA-838-T 32 assays
EUR 421
Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

QuickTiter Lentivirus Titer Kit (Lentivirus-Associated HIV p24), Trial Size

VPK-107-T 32 assays
EUR 409
Description: Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present. Our QuickTiter Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.

QuickTiter Adenovirus Titer Immunoassay Kit, Trial Size

VPK-109-T 20 assays
EUR 374
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells.

ViraBind AAV Purification Kit, Trial Size

VPK-140-T 2 preps
EUR 380
Description: Purification of adeno-associated virus (AAV) using ultracentrifugation is tedious and time-consuming, and yields can be low. Our ViraBind AAV Purification Kits use a single-step proprietary purification matrix, followed by further purification and concentration with a centrifugal concentrator. The result is a higher viral yield with exceptional purity in a fraction of the time.

QuickTiter AAV Quantitation Kit, Trial Size

VPK-145-T 4 assays
EUR 380
Description: Traditionally, adeno-associated virus (AAV) particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability. Our QuickTiter AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV from any serotype.

CytoSelect 24-Well Wound Healing Assay, Trial Size

CBA-120-T 6 assays
EUR 357
Description: Traditionally scratch assays have been used to study cell migration, cell proliferation and wound healing. However, these assays lack a consistently defined wound gap and can result in high inter-sample variation. Our CytoSelect 24-Well Wound Healing Assay provides a much more consistent method to measure cell migration across a wound field gap in vitro. Proprietary inserts generate a consistent 0.9mm wound gap between the cells. Cells may then be treated or monitored for proliferation or migration across the wound field by imaging samples at fixed time points or by time-lapse microscopy.

CytoSelect 96-Well In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation), Trial Size

CBA-150-T 24 assays
EUR 403
Description: Traditionally, the soft agar colony formation assay has been used to monitor anchorage-independent growth. Cells proliferate for 3-4 weeks in a semisolid culture medium, followed by tedious manual counting of colonies. Our CytoSelect 96-Well In Vitro Tumor Sensitivity Assay provides a stringent, anchorage-independent model for chemosenstivity testing and screening of potential anticancer drugs. After just 6-8 days, cell colonies are quantified using a standard colorimetric microplate reader.

CytoSelect 96-Well Phagocytosis Assay(Zymosan Substrate), Trial Size

CBA-224-T 20 assays
EUR 386
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-Well Cellular Senescence Assay Kit (SA-?-gal Activity, Fluorometric Format), Trial Size

CBA-231-T 24 assays
EUR 345
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

GFP ELISA Kit, Trial Size

AKR-121-T 32 assays
EUR 392
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

CaMK2-beta/gamma/delta Colorimetric Cell-Based ELISA Kit

EKC1079 100ul
EUR 572

T-Pro Plasmid Mini Kit (100)

RB94-YPD100 100preps/Kit
EUR 161

T-Pro Plasmid Mini Kit (250)

RB94-YPD250 250preps/Kit
EUR 222

T-Pro Plasmid Midi Kit (20)

RB94-YPI020 20preps/Kit
EUR 213

T-Pro Plasmid Maxi Kit (10)

RB94-YPM010 10preps/Kit
EUR 222

T-Pro EZ Gel Solution 8%

JB02-B008M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 8%

JB02-B008S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 10%

JB02-B010M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 10%

JB02-B010S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 12%

JB02-B012M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 12%

JB02-B012S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 15%

JB02-B015M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 15%

JB02-B015S 100ml/BT
EUR 135

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 152
The related SNPs mapped in or close to genes associated to mobile signaling, lipid metabolism and blood stress homeostasis, and two of the areas have been related to coronary artery illness (P < 0.006) within the Coronary Artery Illness Genome-Extensive Replication and Meta-Evaluation (CARDIoGRAM) consortium. Our findings could present new perception into pathways resulting in subclinical atherosclerosis and subsequent cardiovascular occasions.

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