Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors.

Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors.

The research of mobile processes and gene regulation in terminal erythroid improvement has been drastically facilitated by the technology of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HUDEP-2 cells. The power to effectively genome edit HUDEP-2 cells and make clonal strains vastly expands their utility because the insertion of […]