Speciation of platinum nanoparticles in different cell culture media by HPLC-ICP-TQ-MS and complementary techniques: A contribution to toxicological assays

Toxicological research of nanoparticles (NPs) are extremely demanded these days however they’re very difficult. Within the in vitro assays, the understanding of the function of cell tradition media is essential to derive a correct interpretation of the toxicological outcomes and to take action, new analytical instruments are needed. On this context, an analytical technique based mostly on reversed-phase liquid chromatography hyphenated to inductively coupled plasma-triple quadrupole mass spectrometry (HPLC-ICP-TQ-MS) has been developed for the primary time for the detection and characterization of each 5 and 30 nm PtNPs, in addition to ionic platinum species, in generally used cell tradition media. For this goal, Dulbecco’s Modified Eagle Medium, DMEM-high glucose, DMEM-F12, DMEM 31053-028, and Roswell Park Memorial Institute, RPMI-1640 (supplemented with 10% fetal bovine serum (FBS) and antibiotics) at a number of incubation occasions (24, 48, and 96 h at 37 °C) have been examined.
After a cautious optimization and analytical efficiency, the developed methodology permits to concurrently examine the oxidation course of, resulting in the discharge of ionic species, and the rise within the hydrodynamic quantity of PtNPs, most likely associated to the formation of recent organic entities (protein corona). The magnitude of each processes was discovered to be depending on the examined cell tradition media and incubation occasions. Dynamic mild scattering (DLS) and high-resolution scanning electron microscopy (HR-SEM) have been used as complementary strategies to check the essential strategy of each delicate and exhausting protein corona formation. The feasibility of the HPLC-ICP-TQ-MS to get related info for toxicological research has been demonstrated and in mild of our outcomes, the affect of the cell tradition media on the habits of PtNPs shouldn’t be underestimated.

Three-dimensional tradition methodology enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine continual colitis mannequin

Tonsil-derived mesenchymal stem cells (TMSCs) confirmed therapeutic results on acute and continual murine colitis fashions, owing to their immunomodulatory properties; subsequently, we evaluated enhanced therapeutic results of TMSCs on a murine colitis mannequin utilizing three-dimensional (3D) tradition methodology. The expression of angiogenic components, VEGF, and anti inflammatory cytokines, IL-10, TSG-6, TGF-β, and IDO-1, was considerably larger within the 3D-TMSC-treated group than within the 2D-TMSC-treated group (P < 0.05). At days 18 and 30 after inducing continual colitis, illness exercise index scores have been estimated to be considerably decrease within the 3D-TMSC-treated group than within the colitis management (P < 0.001 and P < 0.001, respectively) and 2D-TMSC-treated teams (P = 0.022 and P = 0.004, respectively).
Body weight loss was considerably decrease within the 3D-TMSC-treated group than within the colitis management (P < 0.001) and 2D-TMSC-treated teams (P = 0.005). Colon size shortening was considerably recovered within the 3D-TMSC-treated group in comparison with that within the 2D-TMSC-treated group (P = 0.001). Histological scoring index was considerably decrease within the 3D-TMSC-treated group than within the 2D-TMSC-treated group (P = 0.002). These outcomes point out that 3D-cultured TMSCs confirmed significantly larger therapeutic results in a continual murine colitis mannequin than these of 2D-cultured TMSCs through elevated anti-inflammatory cytokine expression.
ncbcs
ncbcs

Characterization of the Main Human Trophoblast Cell Secretome Utilizing Steady Isotope Labeling With Amino Acids in Cell Tradition

The placental villus syncytiotrophoblast, the nutrient-transporting and hormone-producing epithelium of the human placenta, is a essential regulator of fetal growth and maternal physiology. Nevertheless, the identities of the proteins synthesized and secreted by main human trophoblast (PHT) cells stay unknown. Steady Isotope Labeling with Amino Acids in Cell Tradition adopted by mass spectrometry evaluation of the conditioned media was used to determine secreted proteins and procure details about their relative charges of synthesis in syncytialized multinucleated PHT cells remoted from regular time period placental villus tissue (n = 4/impartial placenta).
A complete of 1,344 proteins have been recognized, most of which haven’t beforehand been reported to be secreted by the human placenta or trophoblast. The vast majority of secreted proteins are concerned in power and carbon metabolism, glycolysis, biosynthesis of amino acids, purine metabolism, and fatty acid degradation. Histone household proteins and mitochondrial proteins have been amongst proteins with the slowest synthesis fee whereas proteins related to signaling and the plasma membrane have been synthesized quickly.
There was a big overlap between the PHT secretome and proteins identified be secreted to the fetal circulation by the human placenta in vivo. The generated information will information future experiments to find out the perform of particular person secreted proteins and can assist us higher perceive how the placenta controls maternal and fetal physiology.
Pir ELISA Kit| Rat Pirin ELISA Kit
Lifescience Market
REN ELISA Kit| Rat Renin ELISA Kit
Lifescience Market
Kl ELISA Kit| Rat Klotho ELISA Kit
Lifescience Market
LN ELISA Kit| Rat Laminin ELISA Kit
Lifescience Market
Lep ELISA Kit| Rat Leptin ELISA Kit
Lifescience Market

Technology, characterization, and drug sensitivities of 12 patient-derived IDH1-mutant glioma cell cultures

Background: Mutations of the isocitrate dehydrogenase (IDH) gene happen in over 80% of low-grade gliomas and secondary glioblastomas. Regardless of appreciable efforts, endogenous in vitro IDH-mutated glioma fashions stay scarce. Availability of those fashions is essential for the event of recent therapeutic interventions.
Strategies: Cell cultures have been established from recent tumor materials and expanded in serum-free tradition media. D-2-Hydroxyglutarate ranges have been decided by mass spectrometry. Genomic and transcriptomic profiling have been carried out on the Illumina Novaseq platform, methylation profiling was carried out with the Infinium MethylationEpic BeadChip array. Mitochondrial respiration was measured with the Seahorse XF24 Analyzer. Drug screens have been carried out with an NIH FDA-approved anti-cancer drug set and two IDH-mutant particular inhibitors.
Outcomes: A set of twelve patient-derived IDHmt cell cultures was established. We confirmed excessive concordance in driver mutations, copy numbers and methylation profiles between the tumors and derived cultures. Homozygous deletion of CDKN2A/B was noticed in all cultures. IDH-mutant cultures had decrease mitochondrial reserve capability. IDH-mutant particular inhibitors didn’t have an effect on cell viability or international gene expression. Screening of 107 FDA-approved anti-cancer medication recognized 9 compounds with potent exercise in opposition to IDHmt gliomas, together with three compounds with favorable pharmacokinetic traits for CNS penetration: teniposide, omacetaxine mepesuccinate, and marizomib.
Conclusions: Our twelve IDH-mutant cell cultures present excessive similarity to the parental tissues and supply a novel instrument to check the biology and drug sensitivities of high-grade IDHmt gliomas in vitro. Our drug screening research reveal lack of sensitivity to IDHmt inhibitors, however sensitivity to a set of 9 accessible anti-cancer brokers.
Key phrases: IDH1; drug repurposing; glioma; patient-derived cell tradition; preclinical fashions.
Paraffin Wax Dispenser
HIS7000
Paraffin wax, granular (56 - 60)
GL4115-1KG
Paraffin wax, granular (56 - 60)
GL4115-5KG
8KG Histoplast PE Paraffin
HIS3324
Paraffin oil, BP, Ph. Eur. grade
GL4141-1L

In vitro Co-tradition of Mesenchymal Stem Cells and Endothelial Colony Forming Cells

The invention of endothelial colony forming cells (ECFCs) with strong self-renewal and de novo vessel formation potentials means that ECFCs may be a wonderful cell supply for cardiovascular illnesses therapy by bettering neovascularization within the ischemic tissues. Nevertheless, their engraftment after transplantation resulted to be low. Earlier research confirmed mesenchymal stem/stromal cells (MSCs) might enhance the survival and capillary formation capability of ECFCs in co-culture programs. On this article, we describe a protocol for in vitro co-culture of MSCs and ECFCs to prime ECFCs for higher engraftment.

Isolation, Tradition and Differentiation of Grownup Hippocampal Precursor Cells

There are two neurogenic niches within the grownup mammalian mind: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas may be remoted and maintained in vitro, utilizing two completely different tradition programs to evaluate their potential concerning proliferation and differentiation in a reductionist mannequin. Whereas the neurosphere assay is primarily carried out to instantly examine the proliferative and differentiation potential of cells in particular person brains, the monolayer tradition permits single cell evaluation in a moderately homogeneous cell inhabitants. Right here, we describe the isolation, culturing strategies and differentiation of neural precursor cells in each programs.
Pir ELISA Kit| Rat Pirin ELISA Kit
EF019186
REN ELISA Kit| Rat Renin ELISA Kit
EF017245
Kl ELISA Kit| Rat Klotho ELISA Kit
EF017474

Improved Differentiation of hESC-Derived Pancreatic Progenitors by Utilizing Human Fetal Pancreatic Mesenchymal Cells in a Micro-scalable Three-Dimensional Co-tradition System

Mesenchymal cells of numerous origins differ in gene and protein expression in addition to producing various results on their organ-matched epithelial cells’ upkeep and differentiation capability. Co-culture with rodent’s tissue-specific pancreatic mesenchyme accelerates proliferation, self-renewal, and differentiation of pancreatic epithelial progenitors.
Subsequently, in our examine, the influence of three-dimensional (3D) co-culture of human fetal pancreatic-derived mesenchymal cells (hFP-MCs) with human embryonic stem cell-derived pancreatic progenitors (hESC-PPs) growth in the direction of endocrine and beta cells was assessed. Moreover, the power to take care of scalable cultures combining hFP-MCs and hESC-PPs was investigated. hFP-MCs expressed many markers in widespread with bone marrow-derived mesenchymal stem cells (BM-MSCs).
Nevertheless, they confirmed larger expression of DESMIN in comparison with BM-MSCs. After co-culture of hESC-PPs with hFP-MCs, the pancreatic progenitor (PP) spheroids generated in Matrigel had larger expression of NGN3 and INSULIN than BM-MSCs co-culture group, which reveals an inductive influence of pancreatic mesenchyme on hESC-PPs beta-cells maturation.
Pancreatic aggregates generated by compelled aggregation by scalable AggreWell system confirmed comparable options in comparison with the spheroids. These aggregates, a mix of hFP-MCs and hESC-PPs, may be utilized as an applicable instrument for assessing endocrine-niche interactions and developmental processes by mimicking the pancreatic tissue.

Murashige and Skoog, With Gamborg's Vitamins

CP029-500 50L
EUR 165.6

BME 100X Vitamins for Basal Medium Eagle (Modified)

BML01-100ML 100 ml
EUR 87.6
Description: 100X Vitamins for Basal Medium Eagle (Modified)

BME 100X Vitamins for Basal Medium Eagle (Modified)

BML01-500ML 500 ml
EUR 110.4
Description: 100X Vitamins for Basal Medium Eagle (Modified)

Gamborg's B-5 Medium

CP009-010 10X1L
EUR 118.8

Gamborg's B-5 Medium

CP009-500 50L
EUR 151.2

Gamborg's B-5 Long Medium

CP013-010 10X1L
EUR 135.6

Gamborg's B-5 Long Medium

CP013-100 10L
EUR 121.2

Gamborg's B-5 Medium; With 20g/L Sucrose

CP010-010 10X1L
EUR 124.8

Gamborg's B-5 Medium; With 20g/L Sucrose

CP010-500 50L
EUR 141.6

Gamborg's Vitamin Stcks, Liquid

CP049-010 125 ml bottle
EUR 128.4

MURASHIGE SKOOG AGAR W/ VITAMINS

M13-148-10kg 10 kg Ask for price

MURASHIGE SKOOG AGAR W/ VITAMINS

M13-148-2kg 2kg Ask for price

MURASHIGE SKOOG AGAR W/ VITAMINS

M13-148-500g 500 g Ask for price

100mL MEM Vitamins 100x Solution

25-020-CI PK6
EUR 115.2

Murashige and Skoog, With Vitamins

CP030-010 10X1L
EUR 118.8

Murashige and Skoog, With Vitamins

CP030-500 50L
EUR 151.2

Murashige and Skoog, With Vitamins and Glycine

CP031-010 10X1L
EUR 118.8

Murashige and Skoog, With Vitamins and Glycine

CP031-500 50L
EUR 151.2

Morpheus III - 15% Vitamins mix, 50ml *SHIPPED ON ICE*

M-MD2-50-314 each
EUR 250
Description: Morpheus III - 15% Vitamins mix, 50ml *SHIPPED ON ICE*

Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose

CP032-010 10X1L
EUR 121.2

Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose

CP032-500 50L
EUR 151.2

DC MEDIUM W/ BCIG

D04-116-10kg 10 kg
EUR 5446.8

DC MEDIUM W/ BCIG

D04-116-2Kg 2 Kg
EUR 1231.2

DC MEDIUM W/ BCIG

D04-116-500g 500 g
EUR 378

Vitamin B5 [HRP]

DAGA-123H 1mg
EUR 1466.4

Vitamin B5 [KLH]

DAGA-123K 1mg
EUR 1482

Schneider's Medium, w/ L-glutamine

CCM1318-500 500 mL
EUR 104.33

500mL MEM Alpha Medium w salt

15-012-CV PK6
EUR 193.2

500mL MEM Alpha Medium w salts

10-022-CV PK6
EUR 243.6

Human Vitamin B5(VB5)

QY-E05699 96T
EUR 433.2

Medium 199, with L-Glutamine, w/ Earle's salts 

CCM2441-500 500 mL
EUR 81.68

Medium 199

C0012-01 RT 500 mL Bottle
EUR 126

D-Pantothenic acid hemicalcium salt (Vitamin B5)

PB0683 100g
EUR 82.97

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

A Medium

DJ1018 100g
EUR 101.76

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

OF Medium

MED1664 500G
EUR 79.2

M9 Medium

SD7024 250g
EUR 86.1

YM Medium

SD7031 250g
EUR 84.54

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM

H08-107-500g 500 g
EUR 218.4

M63 Medium

SD7033 250g
EUR 86.1

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM

S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM

S19-110-500g 500 g
EUR 128.4

SOB MEDIUM

S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM

S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM

S19-124-500g 500 g
EUR 124.8

Ec Medium 500gm

231430 EACH
EUR 176.4

TCBS Medium

MED1188 PK10
EUR 9.6

M9CA Medium

SD7025 250g
EUR 86.1

Sob Medium 500gm

244310 EACH
EUR 261.6

DNase Medium

MED1196 PK10
EUR 10.8

TYGPN Medium

SD7032 250g
EUR 84.01

Tyvek 500 Xpert Classic disposable Coverall c/w hood Medium

SAF4086 PK100
EUR 1280.4

Heller's Medium

CP014-010 10X1L
EUR 118.8

Heller's Medium

CP014-500 50L
EUR 151.2

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Mounting Medium

4300 3 ml
EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 123.6

COLIFORM MEDIUM

C03-127-10kg 10 kg
EUR 1588.8

COLIFORM MEDIUM

C03-127-2kg 2kg
EUR 392.4

COLIFORM MEDIUM

C03-127-500g 500 g
EUR 150

Hoagland's Medium

CP015-010 10X1L
EUR 118.8

Hoagland's Medium

CP015-500 50L
EUR 151.2

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price

RPMI 1640 Medium

abx098879-500ml 500 ml
EUR 210

A-1 MEDIUM

A01-116-10kg 10 kg
EUR 1240.8

A-1 MEDIUM

A01-116-2Kg 2 Kg
EUR 316.8

A-1 MEDIUM

A01-116-500g 500 g
EUR 129.6

MCDB 131 Medium

CM034-050 500ml
EUR 102

MCDB 131 Medium

CM034-300 6x500ml
EUR 198

MCDB 131 Medium

CM034-310 10x500ml
EUR 262.8

MCDB 131 Medium

CM034-320 20x500ml
EUR 408

MCDB 131 Medium

CM034-350 50x500ml
EUR 702

Medium 199/BCDB 105

C0012-02 RT 500 mL Bottle
EUR 181.2

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)

ABP-MED-10004 1 liter Ask for price

Isp Medium 2 500gm

277010 EACH
EUR 356.4

Isp Medium 4 500gm

277210 EACH
EUR 340.8

BPS Medium 1

79259 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway)

BPS Medium 7

79260 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits.

BPS Medium 8

79385 100 ml
EUR 120
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379).

Thaw Medium 8

79652-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 8

79652-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 9

79665-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D_• CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 9

79665-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, using • CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 10

79704-1 100 ml
EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 10

79704-2 500 ml
EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 11

79976-1 100 ml
EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 11

79976-2 500 ml
EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 12

78074-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 12

78074-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 14

78397-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 14

78397-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15

78412-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15

78412-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 16

78647-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 16

78647-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 4

60181-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 4

60181-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 5

60182-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 5

60182-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 6

60183-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 6

60183-2 500 ml
EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 2

60184-1 100 ml
EUR 150
Description: This liquid Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's Jurkat-based cell lines, including• PD-1/NFAT Reporter-Jurkat cell #60535• NFAT Reporter (Luc) - Jurkat Cell Line #60621• NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540• ADCC Bioassay Effector Cell V variant (High Affinity) #60541Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 2

60184-2 500 ml
EUR 675
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D__x000D_• PD-1/NFAT Reporter-Jurkat cell #60535_x000D__x000D_• NFAT Reporter (Luc) - Jurkat Cell Line #60621_x000D__x000D_• NF-ĸB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651_x000D__x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540_x000D__x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) #60541_x000D__x000D_Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 7

60185-1 100 ml
EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623
Sources :
1. NCBI

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