Rational Laboratory Diagnostics of Antiphospholipid Antibodies: Anti-Cardiolipin, Anti-β2-Glycoprotein I, Anti-Prothrombin and Anti-Annexin V Antibodies.

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A attainable co-appearance of anticardiolipin (aCL), anti-β2-glycoprotein I (anti-β2-GPI), anti-prothrombin (aPT) and anti-annexin V (aANXV) antibodies of IgG, IgM and IgA class have been studied in 58 sufferers with SLE alone and 32 sufferers APS within the view of rational laboratory diagnostics.
The presence of anti-phospholipid antibodies (aPL) have been outlined by our in-house ELISA strategies. Out of 17 aCL destructive SLE sufferers 6 had different antigenically outlined aPL antibodies. In 13 sufferers solely IgA however not IgG and IgM anti-β2GPI have been detected. Totally different mixtures of aPL subsets have been equally distributed in APS and SLE teams.
The prevalence of aANXV have been comparable in APS and SLE sufferers which was not the case with different aPL. Our findings assist the concept of measuring further subsets of aPL (aPT and aANXV) in unclear instances. IgA (both aCL or anti-β2-GPI) improved neither the diagnostic specificity nor diagnostic sensitivity, however solely elevated the frequency of the full anti-β2-GPI.
Vaso-occlusive disaster (VOC) is a big explanation for morbidity and mortality in sickle cell anemia (SCA) sufferers; nevertheless, its mechanisms are poorly understood. In view of their prothrombotic nature, we hypothesized that SCA-associated VOC could also be because of the presence of anti-annexin V antibodies. Anti-annexin V antibodies have been measured with ELISA in 177 VOC and 81 steady-state SCA sufferers.
Anti-annexin V IgM and IgG concentrations have been considerably larger in VOC sufferers than in steady-state sufferers and have been related to elevated VOC danger. After categorizing anti-annexin V antibodies, the adjusted odds ratio elevated because the percentile worth elevated. Monovariate logistic regression evaluation demonstrated a optimistic dose-effect relationship for anti-annexin V IgM with VOC, with elevated VOC danger seen with elevated antibody titers.
Multivariate logistic regression analyses confirmed the affiliation of anti-annexin V IgM, extra so than IgG, as an unbiased VOC danger issue. Anti-annexin V IgG antibodies correlated positively with VOC kind and negatively with HbF and age of VOC onset, whereas anti-annexin V IgM correlated positively with VOC kind, length, frequency, web site, ache severity, hospitalization, and medicine, and negatively with age of VOC onset and HbS ranges. Excessive ranges of anti-annexin V IgM antibodies represent a danger issue for VOC in SCA sufferers.
Rat Cholesterol ELISA ELISA
E01A11128
Goat Cholesterol ELISA ELISA
E01A46041
Mouse Cholesterol ELISA ELISA
E01A19869

Scientific significance of serum anti-annexin V antibodies in Egyptian sufferers with scleroderma.

The pathogenesis of scleroderma encompasses vascular, immunological, and fibrotic processes, which contribute to medical manifestations. We investigated the prevalence of anti-annexin V IgG and IgM antibodies in sera of scleroderma sufferers and their relation to the presence of different antibodies and improvement of illness morbidity.
Sera of 40 scleroderma sufferers and 15 wholesome controls have been examined for IgG and IgM anti-annexin V antibodies by ELISA and anticentromere antibodies by oblique immunofluorescence. Serum stage of anti-annexin V IgG antibodies in scleroderma sufferers was considerably larger than that of the management (P < 0.001) and correlated considerably with the presence of digital ischemia (P = 0.023) and pulmonary fibrosis (P = 0.02).
IgM isotype was comparable between sufferers and controls (P = 0.317). Anticentromere antibodies are extra prevalent within the restricted cutaneous subtype (P = 0.017). In conclusion, measurement of serum anti-annexin V IgG antibodies in scleroderma sufferers could also be necessary for early prognosis of vascular and pulmonary issues.

Induction of apoptosis by cross-linking antibodies certain to human B-lymphoma cells: expression of Annexin V binding websites on the antibody cap.

There are lots of reviews that cross-linking antibodies (Abs) certain to the floor of B-lymphoma cells can induce apoptosis and/or cell dying, particularly with anti-CD20 Abs. This research was supposed to increase our understanding of those results. To find out if CD20 is a novel goal on this respect, or whether or not Abs to different antigens would have comparable results, six Abs have been examined, with and with out cross-linking with a secondary Ab, on three goal cell strains.
We utilized assays that distinguish between apoptotic, lifeless, and viable cells. Two assays have been used: Annexin V plus propidium iodide, and JC-1 plus SYTOX inexperienced (Molecular Probes, Eugene, OR). A lot of the Abs examined induced a low stage of apoptosis and cell dying in Ramos cells, however not within the different two cell strains (Raji and RL).
On the whole, the extent of toxicity was correlated with the extent of antigen expression, with Abs to high-density antigens having the strongest results. Nevertheless, because the majority of Ramos cells continued to multiply, it’s questionable whether or not toxicity at this stage can present a big medical profit. Unexpectedly, there was additionally a inhabitants of cells that stained weakly with Annexin V.
These cells have been distinct from classical apoptotic cells, and appeared to belong to the viable cell inhabitants. In these cells, Annexin V stained the area of the Ab cap, in distinction to the ringed staining of classical apoptotic cells.
Rat Cholesterol ELISA ELISA
E01A11128
Goat Cholesterol ELISA ELISA
E01A46041
Mouse Cholesterol ELISA ELISA
E01A19869

Antibodies to beta2-glycoprotein I and annexin V in ladies with early and late idiopathic recurrent spontaneous abortions.

Anti-phospholipid antibodies (APA) are heterogeneous group of autoantibodies that concentrate on phospholipid or phospholipid-binding proteins. APAs have been beforehand proven to induce a number of thrombotic states, together with idiopathic recurrent spontaneous abortion (RSA). Not like the contribution of the classical lupus anticoagulant (LAC) and anticardiolipin antibodies (ACA), the contribution of anti-beta2 glycoprotein 1 (beta2GPI) and anti-annexin V antibodies to RSA danger stay poorly understood.
We assessed anti-beta2GPI and anti-annexin V IgM and IgG antibodies as RSA danger elements for RSA in 172 Tunisian ladies with >three consecutive idiopathic being pregnant losses, along with 173 matched management ladies. The prevalence of anti-beta2GPI IgG (P=0.41, OR=1.64) and IgM (P=0.50, OR=1.70) have been comparable between instances and controls.

Annexin V Antibody
49376 100ul
EUR 499

Annexin V Antibody
49376-100ul 100ul
EUR 399.6

Annexin V Antibody
49376-50ul 50ul
EUR 286.8

Annexin V Antibody
E2300787 100ug
EUR 225
Description: Available in various conjugation types.

Annexin V Antibody
DF6580 200ul
EUR 420

Annexin V Antibody
DF6580-100ul 100ul
EUR 280

Annexin V Antibody
DF6580-200ul 200ul
EUR 350

Annexin V antibody
E39-09988 100ug/100ul
EUR 225
Description: Available in various conjugation types.

Annexin V Antibody
E300518 100ug/200ul
EUR 275
Description: Available in various conjugation types.

Annexin V antibody
CAF50033-100ug 100ug
EUR 312

Annexin V antibody
CAF50425-100ug 100ug
EUR 338

Annexin V antibody
70R-13691 100 ug
EUR 519
Description: Affinity purified Rabbit polyclonal Annexin V antibody

Annexin V antibody
70R-11770 100 ug
EUR 447
Description: Rabbit polyclonal Annexin V antibody

Annexin V Antibody
F49706-0.08ML 0.08 ml
EUR 140.25
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Annexin V Antibody
F49706-0.4ML 0.4 ml
EUR 322.15
Description: Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Annexin V Antibody
F49707-0.08ML 0.08 ml
EUR 140.25
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]

Annexin V Antibody
F49707-0.4ML 0.4 ml
EUR 322.15
Description: Annexin 5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]

Annexin V Antibody
GWB-70457E 0.1 mg Ask for price

Annexin V Antibody
GWB-BA41FA 0.1 mg Ask for price

Annexin V Antibody
R30176 100 ug
EUR 356.15
Description: Annexin V is also know as endonnexin II (ENX2),or placental protein 4 (PP4). It is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. Annexin V has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. It is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Annexin V Antibody
R31475 100 ug
EUR 356.15
Description: Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Annexin V Antibody
RQ5261 100ul
EUR 356.15
Description: The protein encoded by the ANXA5 gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. [RefSeq]

Annexin V Antibody
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Annexin V Antibody
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MBS852324-01mLAF405L 0.1mL(AF405L)
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MBS852324-01mLAF405S 0.1mL(AF405S)
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MBS852324-01mLAF610 0.1mL(AF610)
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MBS852324-01mLAF635 0.1mL(AF635)
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Annexin V Antibody
MBS5311273-01mg 0.1mg
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MBS5311273-5x01mg 5x0.1mg
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MBS5312528-01mg 0.1mg
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MBS5312528-5x01mg 5x0.1mg
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Annexin V Antibody
MBS9436121-005mL 0.05mL
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Annexin V Antibody
MBS9436121-01mL 0.1mL
EUR 435

Annexin V Antibody
MBS9436121-5x01mL 5x0.1mL
EUR 1810

Annexin V antibody
PAab09988 100ug
EUR 275
Increased anti-annexin V IgG (P=0.02, OR=5.28), however not IgM (P=0.25, OR=1.78), ranges have been seen in instances. Regression evaluation confirmed that anti-beta2GPI IgM (OR=8.90; 95% CI=1.23-64.63) was related to early RSA, whereas anti-annexin V IgG (OR=9.35, 95% CI=1.44-60.86) was related to late RSA. For mixed early + late RSA, the one variable chosen was BMI (OR=0.93; 95% CI=0.87-0.99), and neither anti-annexin V nor anti-beta2GPI IgM and IgG have been related to early + late RSA. Anti-annexin V and anti-beta2GPI seem like unbiased danger markers of RSA.

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